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Registros recuperados: 92
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Analysis of shell colour determinism in the Manila clam Ruditapes philippinarum ArchiMer
Peignon, Jean-marie; Gerard, Andre; Naciri, Yamama; Ledu, Christophe; Phelipot, Pascal.
The Manila clam presents a large polymorphism for shell colours and patterns. No experiment has been conducted to study the determinism of such a polymorphism which has been well known for a long time. The aim of this study was firstly to determine the genetic variation for shell colour and secondly to produce some homozygous lines for the shell colouring. These lines could then be used as controls for laboratory experiments. The colouring is defined by three characters: asymmetry, ornamentation and hue. Clams with asymmetrical colouring are not fully pigmented, except for a posterior band on the left valve. Clams with symmetrical colouring are fully pigmented on the two valves. Four cross series were conducted between the different characters: Asymmetry...
Tipo: Text Palavras-chave: Mollusque; Bivalve; Palourde; Ruditapes philippinarum; Génétique; Hérédité mendélienne; Coloration; Asymétrie; Mollusc; Bivalve; Manila clam; Ruditapes philippinarum; Genetics; Mendelian inheritance; Colouration; Asymmetry.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/1995/publication-3204.pdf
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Rapport d'activité 1999 du Laboratoire Génétique et Pathologie de La Tremblade ArchiMer
Gerard, Andre.
Rapport d'activité 1999 du Laboratoire Génétique et Pathologie de La Tremblade.
Tipo: Text Palavras-chave: Pathologie; Génétique; Invertébrés marins; Rapport Activité.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00071/18261/15824.pdf
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Acquis biologique sur le pétoncle noir (Chlamys varia) en Rade de Brest 1973-1983. Conséquences sur l'aménagement de la ressource ArchiMer
Dao, Jean-claude; Gerard, Andre; Buestel, Dominique.
Research on the black scallop followed that on the king scallop. Indeed, over the 1973-1975 " spadework » period, it has been demonstrated that the roadsted of Brest was: - an overexploited king scallop deposit; the broodstock not being able to ensure a satisfying renewal of the species. - a black scallop production deposit (hundreds of tonnes). In parallel, the collection trials provided null results for the king scallop but very interesting results for the black scallop (thousands of spats per collector). From 1976 onwards, the spat collecting operations did not focus on king scallop juveniles anymore, but on black scallop.
Tipo: Text Palavras-chave: Reproduction; Captage; Physiologie; Pétoncle; Chlamys varia.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/acte-6538.pdf
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Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults ArchiMer
Arzul, Isabelle; Renault, Tristan; Thebault, Anne; Gerard, Andre.
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of...
Tipo: Text Palavras-chave: Protein; DNA; Healthy carrier; Adult oyster; Crassostrea gigas; Herpesvirus.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-697.pdf
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Polyploïdisation chez les mollusques bivalves : techniques d'obtention, performances des produits obtenus - Première partie : maîtrise des techniques de polyploïdisation application aux espèces d'intérêt commercial en France (Huîtres creuses et palourdes) ArchiMer
Gerard, Andre.
Following the development of the hatchery tool, different triploidy induction techniques have been tried out in vitro. The method using cytochalasin B was selected and successfully applied to cupped oysters, Crassostrea gigas and especially to clams, Ruditapes philippinarum. Larval survival rates were lower than control rates. On the other hand, larvae's growth speed was comparable. These methods will now be used to produce significant test populations which will then be cultivated in French coastal waters.
Tipo: Text Palavras-chave: Palourdes; Huîtres creuses; Polyploïdisation; Conchyliculture.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/rapport-3853.PDF
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The use of microsatellite markers for parentage analysis in the Pacific cupped oyster, Crassostrea gigas (Thunberg) ArchiMer
Boudry, Pierre; Collet, Bertrand; Kotoulas, Georgios; Magoulas, Antonio; Hervouet, Veronique; Bonhomme, Francois; Gerard, Andre.
Highly polymorphic genetic markers can be useful tools for the analysis of parental contributions. The present paper reports 3 experiments using microsatellite markers to analyse parental contributions in controlled in vitro factorial crosses of the cupped oyster Crassostrea gigas. Parentage analysis was eased by the large polymorphism observed at the 3 loci studied. In a cross between 20 males and 20 females, a quarter of the progeny could be assigned to a given parent using a single microsatellite locus showing 28 alleles. In 3 crosses between 5 males and females, all the progeny could be assigned to a family by using 3 loci. In all cases, the combined genotyping at 2 loci allowed parentage to be detennined unambiguously. Despite the balanced gametic...
Tipo: Text Palavras-chave: Crassostrea gigas; Microsatellites; Parental contributions; Crassostrea gigas; Microsatellites; Contributions parentales.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/acte-2739.pdf
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Obtention de souches conchylicoles performantes par polyploïdisation (2ème partie) ArchiMer
Gerard, Andre.
Following a phase of experimental system development, acquisition and improvement of triploidisation techniques carried out in 1989, the 1990 program divides into four main actions:1) significant production of diploid and triploid populations of cupped oysters and Pacific clams in order to compare their performances in French coastal waters2) first trials of triploidy induction in European clam3) development of a ploidy control technique at any stage of the animal life, through digital imaging4) summary of the first trials of tetraploidy induction in C. gigas and R. philippinarum
Tipo: Text Palavras-chave: Imagerie numérique; Ruditapes decussatus; Ruditapes philippinarum; Crassostrea gigas; Cytogénétique; Tétraploïdie; Triploïdie; Polyploïdie.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/rapport-3855.PDF
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Influence of parentage upon growth in Ostrea edulis: evidence for inbreeding depression ArchiMer
Naciri-graven, Y; Launey, Sophie; Lebayon, N; Gerard, Andre; Baud, Jean-pierre.
Genetic variability for growth was analysed in three populations of Ostrea edulis, selected for resistance to the protozoan parasite Bonamia ostreae. This study was undertaken first to determine the potential for selection for growth in populations that have never been selected for this character, and second to estimate heterosis versus inbreeding depression. Growth was monitored in culture for 10 months. The selected populations (namely S85-G3, S89I-G2 and S89W-G2), their crossbred population and a control population were composed of full-sib families whose parents were already genotyped using five microsatellite markers. This genotyping allowed the estimation of genetic relatedness among pairs of parents. The parents' relatedness was then correlated with...
Tipo: Text
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-2719.pdf
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Production artificielle de naissain de coquilles Saint-Jacques Pecten maximus (L.) : Analyse des facteurs affectant la croissance larvaire ArchiMer
Cochard, Jean-claude; Gerard, Andre.
The analysis of the Pecten maximus larval productions carried out between 1982 and 1986 in the framework of the repopulating program of the roadsted of Brest demonstrated the existence of a number of factors affecting larval growth in hatcheries: - Larval growth shows important seasonal fluctuations. It is minimal in February-March (3 to 4 µm.D-1) and maximal in June (6 to 8 µm.D-1). The growth of larvae from conditioned spawners does not differ from that of larvae from mature genitors collected in their natural habitat. Growth is favoured by an unknown factor present in sea water and held with a filtration of 0,2 µm. - The growth of the larvae cultivated in slack water in flat bottom ponds is higher than that of the larvae cultivated in bubbling water in...
Tipo: Text Palavras-chave: Larve; Ecloserie; Croissance organisme; Coquille Saint Jacques; Pecten maximus L.; Production.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-6534.pdf
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Les récifs artificiels - Etat des connaissances et recommandations ArchiMer
Gerard, Andre; Thouard, Emmanuel; Veron, Gerard; Denis, Jacques; Thebaud, Olivier.
The "Artificial reefs" and "Repopulating" issues have been tackled by the Ifremer for a number of years without reaching a clear cut point of view, mainly for lack of socio-economic elements necessary to a rigorous analysis of these devices and practices. During a mission in Japan in January 2006, some Japanese experts, have presented to the head of the Ifremer a few examples of realisations related to these fields and conducted in their country. The head of the Ifremer realised the scope of such devices in Japan and expressed the wish to see an in-depth study carried out over there. In this purpose, the International Relationship Department organised and co-financed the three-month stay in Japan of a student from the University of Montpellier III, Sylvain...
Tipo: Text Palavras-chave: France; Etranger; Recommandations; Réglementation; Cadre juridique; Protection; Aménagement; Intérêt; Ecosystème; Récifs artificiels.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/rapport-6533.pdf
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Obtention de souches conchylicoles performantes par polyploïdisation (3ème partie) ArchiMer
Gerard, Andre; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Bodoy, Alain; Heurtebise, Serge; Garnier, Jacqueline.
The third part of the polyploidisation program of the main French species of commercial interest, this report presents the actions and results for the year 1991 which was devoted to:- continuation of the development trials of the triploidy induction technique in Ruditapes decussates (i.e. indigenous clam)- production of diploid and triploid populations of Ostrea edulis (i.e. flat oyster)- control of diploid and triploid population performances in cupped oyster and Pacific clam in their natural habitat- continuation of the development of a ploidy control technique through image analysis- beginning of the development of a new polyploidy induction technique in bivalve molluscs
Tipo: Text Palavras-chave: Ruditapes decussatus; Ostrea edulis; Ruditapes philippinarum; Crassostrea gigas; Imagerie numérique; Cytogénétique; Polyploïdie.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/rapport-3857.PDF
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Production en masse de Skeletonema costatum en bassins extérieurs par fertilisation minérale : étude d'un cycle estival ArchiMer
Gautier, Dominique; Ledu, Christophe; Hussenot, Jerome; Gerard, Andre.
La production en masse de Skeletonemca costatum par fertilisation minérale ((N, P, Si) a été étudiée à l'écloserie IFREMER de Ronce-les-Bains sur un bassin de 200 m3 à fond bâché endant un cycle de 11 jours en juillet 1992. (...)
Tipo: Text Palavras-chave: Phyoplancton; Nutriments; Production en masse; Skeletonema costatum; Diatomées.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00020/13113/10121.pdf
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Repeuplement et soutien aux ressources halieutiques - Etat des connaissances et recommandations ArchiMer
Gerard, Andre; Thouard, Emmanuel; Zambonino, Jose-luis; Gaignon, Jean-louis; Antoine, Loic; Harache, Yves; Veron, Gerard; Perez, Jose.
The repopulating of aquatic environments has long been based on the idea that in order to increase halieutic resources, one just had to artificially increase the number of fish in their early stages in marine or fresh water environments. Therefore, as the knowledge on the reproduction of some species and the mastery of their artificial reproduction was growing, the capacity to produce large amounts of eggs or fries from different fish or mollusc species became a reality. Controlled reproduction and the availability of large quantities of fertilized eggs, and then of larvae showing, in captivity, a greater survival capacity than naturally reproduced ones, lead to an increase of repopulating attempts through the release of fries or young fishes raised in...
Tipo: Text Palavras-chave: Recommandation; Repeuplement; Milieu aquatique; Ressources halieutiques.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/rapport-6532.pdf
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Individual growth variation and its relationship with survival in juvenile Pacific oysters, Crassostrea gigas (Thunberg) ArchiMer
Boudry, Pierre; Collet, B; Mccombie, Helen; Ernande, Bruno; Morand, B; Heurtebise, Serge; Gerard, Andre.
In order to study individual growth variability and its relationship with survival in juvenile Crassostrea gigas, parental oysters were sampled at four sites located along the French Atlantic coast and bred under controlled hatchery conditions. Four groups of larvae were obtained by crossing five males and five females from each of the four sites, and a fifth group by crossing these 20 males and 20 females together in a pool. Larvae were reared under conditions allowing the maintenance of a maximum variability of size and gave five experimental groups. Oysters were individually monitored for growth and survival from 3 to 10 months after fertilization. The individual growth performances were relatively stable over time and no noticeable compensation for...
Tipo: Text Palavras-chave: Survival; Oysters; Growth; Crassostrea gigas; Aquaculture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-467.pdf
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Performance of triploid Pacific oysters Crassostrea gigas (Thunberg) reared in high carrying capacity ecosystem : survival, growth and proximate biochemical composition ArchiMer
Goulletquer, Philippe; Joly, Jean-pierre; Gerard, Andre; Le Gagneur, Eric; Moriceau, Jacques; Peignon, Jean-marie; Heurtebise, Serge; Phelipot, Pascal.
Triploid oysters Crassllstrea gigas were: produced in 1990 by treating fertilized eggs with cytochalasin B (CS). Triploids, treated diploids, and controls were deployed early 1991 in a high carrying capacity ecosystem on the Eastern Coast of Normandy (France). A monthly monitoring in 1992 showed that triploids yielded significantly higher growth rate and biochemical composition. However, growth was more heterogeneous. No evidence was found for a length or weight bimodal frequency distribution within triploid groups after a 26 months rearing cycle. Triploids showed retardation of gametogenesis. Carbohydrates content in triploids remained almost constant (40%) from June to September. Their survival rates were significantly lower than controls. Moreover...
Tipo: Text Palavras-chave: Biochemical compostion; Survival; Carrying capacity; Triploid; Growth; Oysters; Crassostrea gigas; Composition biochimique; Survie; Capacité trophque; Triploïdes; Croissance; Huîtres; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-3088.pdf
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Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) ArchiMer
Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe.
A novel method using 6-dimcthylaminopurinc (6-0MAP) has recently been demonstrated to be a good triploid inductor for bivalve molluscs. The effects of 6-DMAP concentration, timing of treatment after fertilization and treatment duration on survival and triploid induction of Crassostrea gigas (Thunberg) embryos were examined. An influence of sperm concentration was also detected and discussed. Survival to D-stage was inversely related to 6-DMAP concentration and the percentage of triploids was shown to be 6-DMAP dose dependent. Two optimal moments for treatment application were determined corresponding to extrusions of the first and second polar bodies. Optimal treatment of 450 µmol/l 6-DMAP beginning 15 min after fertilization over a 10 min period yielded...
Tipo: Text Palavras-chave: Genetic; Triploid induction; Crassostrea gigas; 6 DMAP; Triploid; Oysters.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-3198.pdf
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Influence of ploidy and metal-metal interactions on the accumulation of Ag, Cd, and Cu in oysters Crassostrea gigas thunberg ArchiMer
Amiard, Jean-claude; Perrein Ettajani, H; Gerard, Andre; Baud, Jean-pierre; Amiard Triquet, C.
The present study was designed to compare the response to contaminants in diploid with triploid specimens of the oyster Crassostrea gigas. The reproduction investment in bivalve molluscs has priority on somatic growth. Thus, genetic sterilization by triploidy induction enables the energy flux to be directed toward somatic growth and glucide storage. Bioaccumulation was examined for Ag (10 mug/L), Cd (10 mug/L), and Cu (30 mug/L) to determine if the response to metals follows similar patterns in diploid (2n) and triploid (3n) groups. The effect of ploidy was also evaluated as a function of dry weight of soft tissue and condition index. Moreover, the reciprocal influence of these metals on their incorporation was studied. The results showed that the major...
Tipo: Text Palavras-chave: Oyster culture; Metal bioaccaumulation; Polyploidy.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-791.pdf
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Is fertility of hybrids enough to conclude that the two oysters Crassostrea gigas and Crassostrea angulata are the same species? ArchiMer
Huvet, Arnaud; Gerard, Andre; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Boudry, Pierre.
The distinction of the two cupped oysters Crassostrea gigas (Thunberg, 1793) and Crassostrea angulata (Lamark, 1819) into two species was chiefly due to their differing geographical distributions, C. gigas being present in Asia and C. angulata in Europe. Today it is commonly accepted that C. angulata and C. gigas are a single species according to morphological, genetic and F1 hybridization data. However, the demonstration of the fertility of their hybrids and the absence of any reproductive isolation remained to be investigated. Consequently, we studied the fertility of hybrids and sperm competition by performing three different experiments and producing G1 and G2 hybrid progenies between wild populations of C. angulata and C. gigas. Progenies showed very...
Tipo: Text Palavras-chave: Crassostrea angulata; Crassostrea gigas; Developmental yields of larvae; Fertility; Controlled crosses; Hybridization.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-1395.pdf
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Programme Coquille Saint-Jacques, filière technique de culture sur le fond. Bilan des contrats de plan 1983-1988 & 1989-1993. 7. Liste des publications et documents internes des programmes pluriannuels coquille Saint Jacques ArchiMer
Gerard, Andre.
Cette revue bibliographique, consacrée à la 'Ifilière" coquille Saint-Jacques, devait en théorie, faire abstraction de toutes les études traitant de cette espèce comme d'un "modèle" biologique. Lu frontière entre "fi1ière1'et "modèle" n'est pas aussi simple que l'on pourrait le penser. Lu filière coquille Saint-Jacques, après les échecs des opérations de captage, repose depuis 1982 sur la production de naissain en écloserie. Le développement d'une telle filière de production ne pouvait se faire sans un minimum de recherche dans des domaines comme la "nutrition larvairen ou la "Iphysiologie de la reproduction1'. Ces domaines de recherche qui se sont développés à I'IFREMER, l'ont été , au moins au départ, pour répondre aux besoins des acteurs de la filière...
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Ano: 1994 URL: http://archimer.ifremer.fr/doc/00080/19080/16675.pdf
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Contrôle bactériologique dans des élevages d'Artemia salina ArchiMer
Gerard, Andre.
Des élevages d'Artemia salina en milieux non renouvelés sont réalisés au Centre Océanologique de Bretagne pour l'étude de la nutrition et le contrôle des taux physiologiques, Sur ces élevages, un certain nombre de paramètres sont mesurés en continu ou semi-continu : pH, température, oxygène dissous, nitrites, ammoniaque, biomasse chlorophylienne... Les premières expériences avaient mis en évidence des variations dans le temps des paramètres mesurés avec des seuils d'équilibre résultant vraisemblablement d'une auto-régulation par les bactéries. Dans tous les écosystèmes aquatiques ou terrestres le rôle de la microflore bactérienne est primordial ; le métabolisme bactérien est un maillon essentiel des cycles vitaux du carbone, de l'azote et du soufre... Nous...
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Ano: 1976 URL: http://archimer.ifremer.fr/doc/00444/55535/57124.pdf
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